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SEC operates also in the preparative way by trapping the lesser molecules within the pores of a particles. The bigger molecules only pass through the pores as They're as well substantial to enter the pores. Larger sized molecules for that reason stream in the column more rapidly than more compact molecules: that may be, the lesser the molecule, the
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The stationary period selectively interacts with diverse sample components, allowing for them to individual as they go through the column.Reverse period HPLC is extensively used in pharmaceutical Examination, in which the separation of drug compounds from advanced matrices is vital.In this technique the columns are filled with polar, inorganic part